Journal: The Journal of Biological Chemistry

Article Title: Laccaic Acid A Is a Direct, DNA-competitive Inhibitor of DNA Methyltransferase 1 *

doi: 10.1074/jbc.M113.480517

Figure Lengend Snippet: qPCR validation of LCA microarray analysis and changes in gene expression caused by treatment with control compound, anthraquinone-2-carboxylic acid

Article Snippet: Microarray Analysis Total RNA was isolated from 2 × 10 5 cells and used for microarray analysis (University of Iowa DNA Core Facility) in hybridization to Human Gene ST1.0 Array GeneChips (Affymetrix).

Techniques: Biomarker Discovery, Microarray, Gene Expression, Control

Journal: Microbiology

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

doi: 10.1099/mic.0.060236-0

Figure Lengend Snippet: qRT-PCR primers used to validate the microarray results

Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Techniques: Microarray

Journal: Microbiology

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

doi: 10.1099/mic.0.060236-0

Figure Lengend Snippet: qRT-PCR validation of microarray data

Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Techniques: Biomarker Discovery, Microarray

Journal: Genome Biology

Article Title: Reverse-engineering the Arabidopsis thaliana transcriptional network under changing environmental conditions

doi: 10.1186/gb-2009-10-9-r96

Figure Lengend Snippet: Predictive power for gene expression of the transcriptional model of A. thaliana inferred from the whole dataset (1,436 conditions) and the test model from 1,292 microarray experiments used as a training set. The left column shows the regression coefficient ( R 2 ) between the model and experimental profiles across the whole dataset for the five best predicted genes. The right column shows R 2 between the test model and the 144 experimental profiles used as the test set for the same five genes. In either case, correlation coefficients were highly significant.

Article Snippet: The data used for the inference procedure were a compendium of 1,436 Affymetrix microarray hybridization experiments publicly available at The Arabidopsis Information Resource (TAIR) website; these were normalized using the robust multi-array average method [ ].

Techniques: Expressing, Microarray